© 2005 European Society of Cardiology
Functional assessment of myoblast transplantation for cardiac repair with magnetic resonance imaging
a Departments of Cardiology, Duke University Medical Center Durham, NC 27710, USA
b Department of Surgery, Duke University Medical Center Durham, NC, USA
c Department of Biomedical Engineering, Duke University Medical Center Durham, NC, USA
d Division of Experimental Cardiology, Thoraxcenter, Erasmus Medical Center Rotterdam, The Netherlands
* Corresponding author. Present address: University of Minnesota, Department of Physiology, 6-125 Jackson Hall, Minneapolis, MN 55455, USA. Tel: +1-612-626-1416; Fax: +1-612-626-1121. E-mail address: dataylor{at}tc.umn.edu
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Abstract |
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Background: Contraction of transplanted myoblasts and their effects on function and remodeling after myocardial infarction remain controversial.
Aim: We used magnetic resonance imaging (MRI) to study wall thickening and left ventricular (LV) function and geometry after myoblast transplantation.
Methods and results: Three weeks after cryo-infarction rabbits were randomized to receive an injection of 
2x108 myoblasts (n=8) or medium (n=9) into the scar. Cine MRI and contrast enhanced (ce) MRI images were acquired before injection (baseline) and 4 weeks later (endpoint). Regional wall thickening was measured at the site of transmural hyperenhancement. In the control group, regional wall thickening decreased to –15.3±8.6% at baseline, which further decreased to –18.3±5.7% at endpoint. Further, end-diastolic volume increased from 3.96±0.27 to 5.00±0.46 ml and end-systolic volume from 2.23±0.19 to 2.96±0.30 ml (both P<0.05 vs. baseline), which was accompanied by increased LV wall volumes (P<0.05 vs. baseline). In contrast, myoblast transplantation increased regional wall thickening from –11.9±15.9% at baseline to 26.9±17.0% (P<0.05 vs. control), which resulted in significantly improved two-dimensional ejection fractions at the infarct level and prevented the increase in end-diastolic and end-systolic volumes and wall volume.
Conclusion: Intracardiac myoblast transplantation after myocardial infarction improves regional wall thickening and prevents progressive left ventricular remodeling.
Key Words: Myocardial infarction • Cellular cardiomyoplasty • Wall thickening • Myoblasts • Magnetic resonance imaging • Stem cells
Received November 13, 2003; Revised December 15, 2003; Accepted December 24, 2003
Orginally submitted to Circulation.
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