© 2005 European Society of Cardiology
Distinct molecular portraits of human failing hearts identified by dedicated cDNA microarrays
Laboratoire de Physiopathologie et de Pharmacologie Cellulaires et Moléculaires INSERM U533, Faculté de Médecine, 1 Rue Gaston Veil, BP 53508, 44035 Nantes, Cedex 1, France
* Corresponding author. Tel.: +33-240412844; fax: +33-240412950. E-mail address: marja.steenman{at}nantes.inserm.fr
 |
Abstract |
|---|
Aims: This study aimed to investigate whether a molecular profiling approach should be pursued for the classification of heart failure patients.
Methods and results: Applying a subtraction strategy we created a cDNA library consisting of cardiac- and heart failure-relevant clones that were used to construct dedicated cDNA microarrays. We measured relative expression levels of the corresponding genes in left ventricle tissue from 17 patients (15 failing hearts and 2 nonfailing hearts). Significance analysis of microarrays was used to select 159 genes that distinguished between all patients. Two-way hierarchical clustering of the 17 patients and the 159 selected genes led to the identification of three major subgroups of patients, each with a specific molecular portrait. The two nonfailing hearts clustered closely together. Interestingly, our classification of patients based on their molecular portraits did not correspond to an identified etiological classification. Remarkably, patients with the highest medical urgency status (United Network for Organ Sharing, Status 1A) clustered together.
Conclusion: With this pilot feasibility study we demonstrated a novel classification of end-stage heart failure patients, which encourages further development of this approach in prospective studies on heart failure patients at earlier stages of the disease.
Key Words: Classification • Idiopathic dilated cardiomyopathy • Coronary artery disease • Gene expression profiling • Cluster analysis
Received March 31, 2004; Revised April 19, 2004; Accepted May 17, 2004
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Chopard, M. Lecunff, R. Danger, G. Lamirault, A. Bihouee, R. Teusan, B. J. Jasmin, J. F. Marini, and J. J. Leger Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures Physiol Genomics, August 7, 2009; 38(3): 291 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Margulies, D. P. Bednarik, and D. L. Dries Genomics, transcriptional profiling, and heart failure. J. Am. Coll. Cardiol., May 12, 2009; 53(19): 1752 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Nevarez, V. Vasseur, G. Le Drean, A. Tanguy, I. Guisle-Marsollier, R. Houlgatte, and G. Barbier Isolation and analysis of differentially expressed genes in Penicillium glabrum subjected to thermal stress Microbiology, December 1, 2008; 154(12): 3752 - 3765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Samuel, M. C. Schaub, M. Zaugg, M. Mamas, W. B. Dunn, and B. Swynghedauw Genomics in cardiac metabolism Cardiovasc Res, July 15, 2008; 79(2): 218 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kuner, A. S. Barth, M. Ruschhaupt, A. Buness, L. Zwermann, E. Kreuzer, G. Steinbeck, A. Poustka, H. Sultmann, and M. Nabauer Genomic analysis reveals poor separation of human cardiomyopathies of ischemic and nonischemic etiologies Physiol Genomics, June 1, 2008; 34(1): 88 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Swynghedauw Phenotypic plasticity of adult myocardium: molecular mechanisms J. Exp. Biol., June 15, 2006; 209(12): 2320 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Beisvag, P. K. Lehre, H. Midelfart, H. Aass, O. Geiran, A. K. Sandvik, A. Laegreid, J. Komorowski, and O. Ellingsen Aetiology-specific patterns in end-stage heart failure patients identified by functional annotation and classification of microarray data Eur J Heart Fail, June 1, 2006; 8(4): 381 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Demolombe, C. Marionneau, S. Le Bouter, F. Charpentier, and D. Escande Functional genomics of cardiac ion channel genes Cardiovasc Res, August 15, 2005; 67(3): 438 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Royer, T. A.B. van Veen, S. Le Bouter, C. Marionneau, V. Griol-Charhbili, A.-L. Leoni, M. Steenman, H. V.M. van Rijen, S. Demolombe, C. A. Goddard, et al. Mouse Model of SCN5A-Linked Hereditary Lenegre's Disease: Age-Related Conduction Slowing and Myocardial Fibrosis Circulation, April 12, 2005; 111(14): 1738 - 1746. [Abstract] [Full Text] [PDF]
|
|