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European Journal of Heart Failure 2003 5(6):733-739; doi:10.1016/S1388-9842(03)00148-X
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© 2003 European Society of Cardiology

Quantitative analysis of peroxisome proliferator-activated receptor gamma (PPAR{gamma}) expression in arteries and hearts of patients with ischaemic or dilated cardiomyopathy

Mohammad R. Mehrabia, Petra Haslmayerb, Susanne Humpelerc,d, Gerhard Strauss-Blaschec,d, Wolfgang Marktlc,d, Forouzan Tamaddona, Nermin Serbecica, Georg Wieselthalere, Theresia Thalhammerb, Helmut D. Glogara and Cem Ekmekciogluc,d,*

a Department of Cardiology, General Hospital (AKH) Währinger Gürtel 18-20, Vienna 1090, Austria
b Department of Pathophysiology, General Hospital (AKH) Währinger Gürtel 18-20, Vienna 1090, Austria
c Department of Physiology, University of Vienna, Faculty of Medicine Schwarzspanierstrasse 17, Vienna A-1090, Austria
d Ludwig Boltzmann Institute for Physiological Rhythms Research Kurzentrum, Bad Tatzmannsdorf A-7431, Austria
e Department of Cardio-Thoracic Surgery, General Hospital (AKH) Währinger Gürtel 18-20, Vienna 1090, Austria

* Corresponding author. Tel.: +43-1-4277-62114; fax: +43-1-4277-62199. E-mail address: cem.ekmekcioglu{at}univie.ac.at


  Abstract

PPAR{gamma}, a nuclear transcription factor, is expressed in various cells within the vasculature and in cardiomyocytes. It has been suggested that PPAR{gamma} is involved in atherogenesis and in cardiac hypertrophy. Therefore, we sought to quantify PPAR{gamma} mRNA in coronary arteries, the aorta and left ventricular specimens from patients with ischaemic (CHD) and dilated cardiomyopathy (CMP). Using real-time PCR, we were able to demonstrate the expression of PPAR{gamma} in all of the human specimens. The lowest expression of PPAR{gamma} was detected in the aorta specimens of both groups (this was set to one). In comparison, the expression in coronary arteries was 2.32-fold in CHD- and 3.78-fold in CMP specimens and in the left ventricle specimens, 2.12-fold in CHD- and 3.51-fold in CMP. Samples from CHD patients showed a higher expression of PPAR{gamma} in all of the samples compared to those from CMP patients (aorta: 1.99-fold; coronary arteries: 1.35; left ventricles: 1.23). PPAR{gamma} levels were not significantly correlated to CD 36 expression values in any group, suggesting that higher levels of PPAR{gamma} are not principally due to increased PPAR{gamma} expression in macrophages. This was confirmed by immunohistochemical analysis, which showed that PPAR{gamma} is also located in the smooth muscle layer and in cardiomyocytes. In conclusion, our observations of increased PPAR mRNA expression in the coronary arteries and left ventricles from CHD and CMP patients suggest an important function of this nuclear receptor in the pathogenesis of heart disease.

Key Words: PPAR{gamma} • Quantitative PCR • Human hearts • Coronary heart disease • Cardiomyopathy

Received August 21, 2002; Revised January 9, 2003; Accepted April 17, 2003


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