Skip Navigation

European Journal of Heart Failure 2008 10(1):47-54; doi:10.1016/j.ejheart.2007.10.013
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Zhao, X.-Y.
Right arrow Articles by Zhu, Z.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, X.-Y.
Right arrow Articles by Zhu, Z.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 European Society of Cardiology

rAAV-asPLB transfer attenuates abnormal sarcoplasmic reticulum Ca2+-ATPase activity and cardiac dysfunction in rats with myocardial infarction

Xiao-Yan Zhaoa, Shen-Jiang Hua,c,*, Jiang Lia, Yun Moua, Ka Bianb,c, Jian Suna and Zhao-Hui Zhua

a Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University 79 Qingchun Road, Hangzhou 310003, China
b Department of Integrative Biology and Pharmacology, The University of Texas-Houston Medical School 6431 Fannin, Houston, TX, 77030, USA
c E-Institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine China

* Corresponding author. Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China. Tel.: +86 571 87236569; fax: 86 571 87236628. E-mail address: s0hu0001{at}hotmail.com


  Abstract

Background: Diminished myocardial sarcoplasmic reticulum Ca2+-ATPase (SERCA) activity and upregulated phospholamban (PLB) level during cardiac dysfunction, had been reported in many studies.

Aims: The current study was designed to examine the effects of rAAV-antisense phospholamban (asPLB) gene transfer on cardiac function, SERCA expression and activity, as well as PLB expression and phosphorylation (Pser16-PLB), in a rat myocardial infarction (MI) model.

Methods and results: Rat MI model was generated by ligating the left anterior descending coronary artery. Four weeks later, left ventricular ejection fraction (LVEF), left ventricular systolic pressure (LVSP), the maximal rates of increase and decrease in intraventricular pressure (±dp/dtmax) were significantly depressed, and left ventricular end diastolic pressure (LVEDP) was increased. Myocardial PLB was markedly increased while both SERCA activity and Pser16-PLB level were decreased. In rAAV-asPLB transfected rats, rAAV-asPLB, which was injected into the myocardium around the infarction area immediately after the coronary artery ligation, effectively attenuated the depression of cardiac function, significantly inhibited the expression of PLB, restored Pser16-PLB level and enhanced myocardium SERCA activity.

Conclusion: rAAV-asPLB transfer in rats with MI effectively prevented the progression of heart failure.

Key Words: Heart failure • Gene therapy • RNA, antisense

Received February 15, 2007; Revised August 22, 2007; Accepted October 19, 2007


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.