© 2006 European Society of Cardiology
Granulocyte Macrophage-Colony Stimulating Factor receptor expression on human cardiomyocytes from end-stage heart failure patients
a Department of Cellular and Molecular Biology and Pathology "L. Califano", "Federico II" University, Via S. Pansini 5, Ed. 19, 80131 Naples, Italy
b Department of Bio-Morphological and Functional Sciences, University "Federico II" Naples Italy
c Department of Cardiothoracic and Respiratory Sciences, Second University of Naples Italy
d Department of Experimental and Clinical Medicine, University "Federico II" Naples Italy
e Inter-University Centre for Cardiovascular Research (G.I.M.E.C.), Second University of Naples Italy
* Corresponding author. Tel.: +39 081 7463016; fax: +39 081 7701016. E-mail address: lorposti{at}unina.it
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Abstract |
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Background: In remodelling ventricles, the progression of heart failure is associated with structural changes involving the extra-cellular matrix (ECM) and the cytoskeleton of cardiomyocytes, associated with fibrosis, cellular damage and death.
The role of some cytokines and haematopoietic growth factors in the mechanism of both damage and regeneration of cardiac tissue during acute myocardial infarction has been demonstrated. Following heart damage, the development of scarred tissue was considered to be the only outcome, since myocytes were considered to be terminally differentiated cells. However, recent studies in animal models and adult human hearts have shown that myocytes can proliferate under the modulation of several factors.
Aims: To assess Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) receptor expression in healthy and diseased human hearts, and to evaluate the possible role of GM-CSF and its receptor in the regeneration of cardiac tissue in chronic cardiomyopathy.
Methods and results: GM-CSFR expression in human cardiac tissue from explanted hearts of ten patients with end-stage heart failure and in cardiac biopsies from eight normal human hearts was studied by immunohistochemistry, and cellular and molecular biology assays.
Our results demonstrated an increase in GM-CSFR in cardiomyocytes from end-stage heart failure tissues as compared to normal control tissues.
Conclusions: We hypothesize that GM-CSF plays a role in apoptotic and/or ECM deposition processes as well as in cytoskeleton modification in the myocardium.
Key Words: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) • GM-CSF receptor • Cardiac regeneration • Ischaemic and dilated cardiomyopathy
Received March 16, 2005; Revised July 20, 2005; Accepted December 7, 2005
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