© 2004 European Society of Cardiology
Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium
a Department of Internal Medicine, Seoul National University College of Medicine and Cardiovascular Research Laboratory Clinical Research Institute, Seoul National University Hospital, Seoul, Korea
b Department of Internal Medicine, College of Medicine Chungbuk National University, Cheongju, Korea
c Medical Research Institute College of Medicine, Chungbuk National University, Cheongju, Korea
d Department of Clinical Pathology College of Medicine, Chungbuk National University, Cheongju, Korea
* Corresponding author. Department of Internal Medicine, College of Medicine, Chungbuk National University, # 62 Gaeshin-dong, Hungduk-gu, Cheongju, 361-711, Korea. Tel.: +82 43 269 6356 fax: +82 43 273 3252. E-mail address: mccho{at}chungbuk.ac.kr
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Abstract |
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Background: Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI.
Methods: Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3x106 cells) or media were epicardially injected into the center and the border area of the infarct scar.
Results: Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric 
-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group.
Conclusion: Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.
Key Words: Cells • Transplantation • Myocardial infarction • Heart failure
Received December 4, 2003; Revised August 2, 2004; Accepted September 23, 2004
1 T.J. Youn and H. Piao contributed equally to this work.
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