The accelerated post-infarction progression of cardiac remodelling is associated with genetic changes in an untreated streptozotocin-induced diabetic rat model
1 Center of Coronary Heart Disease, Cardiovascular Institute and Fu-Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 167 BeiLiShi Road, Beijing 100037, P.R. China
2 Center of Pulmonary Vascular Disease, Cardiovascular Institute and Fu-Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, P.R. China
3 Center of Hypertension, Cardiovascular Institute and Fu-Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, P.R. China
4 School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
* Corresponding author. Tel: +86 10 88398107, Fax: +86 10 68351786, Email: canghailinghu{at}gmail.com (Y.-J.W.); yangyjfw{at}yahoo.com.cn (Y.-J.Y.)
 |
Abstract |
|---|
Aims: The mechanism by which diabetes mellitus exacerbates myocardial injury and the incidence of heart failure after acute myocardial infarction (AMI), remains unclear. We studied the severity of cardiac dysfunction and time-dependent gene expression in a hyperglycaemic rat model with AMI.
Methods and results: The diabetic model was produced by injection of streptozotocin in Sprague-Dawley rats. Ten weeks after induction of diabetes, AMI was induced by ligation of the left anterior descending coronary artery. Cardiac function and left ventricular (LV) dimensions were evaluated using two-dimensional echocardiography. Structural changes were assessed by histological examination. Gene expression profile was documented by using affymetrix genechip U230 2.0 array and real time-PCR. During 56 days post-AMI, lower survival rates, worse LV function, more severe fibrosis, and larger LV diameters were identified in diabetic rats compared with non-diabetic rats. A total 1221 genes involved in processes, such as glucose metabolism, fatty acid metabolism, extracellular matrix, and apoptosis, were found to be differentially expressed between diabetic and non-diabetic rats, of these 770 were up-regulated and 451 down-regulated. Up-regulation of the genes was found 1–2 weeks earlier in diabetic rats than in non-diabetic rats.
Conclusion: The present data suggest that hyperglycaemia up-regulates remodelling-related genes, which may be responsible for the worse outcomes in diabetics than in non-diabetics after AMI.
Key Words: Diabetes mellitus • Acute myocardial infarction • Left ventricular remodelling • Microarray analysis
Received February 4, 2009; Revised July 7, 2009; Accepted July 21, 2009